Final answer:
To determine if a bacterium is an obligate anaerobe, it can be cultured on various media under different oxygen conditions. If there is growth on blood agar incubated anaerobically and no growth on blood agar incubated aerobically, the bacterium can be classified as an obligate anaerobe.
Step-by-step explanation:
Obligate anaerobes are organisms that cannot grow in the presence of oxygen. They are killed by oxygen. To determine if a bacterium is an obligate anaerobe, it can be cultured on various media under different oxygen conditions. If the bacterium grows only in anaerobic conditions (e.g., in an anaerobic jar or tube), it is considered an obligate anaerobe.
Therefore, for the given scenario, the growth or lack of growth of the bacterium on different media and incubation conditions will help determine if it is an obligate anaerobe. If there is growth on blood agar incubated anaerobically and no growth on blood agar incubated aerobically, the bacterium can be classified as an obligate anaerobe. Similarly, if there is growth on chocolate agar incubated in a CO2 incubator.