Question

Gram staining-staining properties of bacteria Gram positive cells primarily hold which dye in the gram stain process?

a) Crystal violet
b) Safranin
c) Iodine
d) Ethanol

Answer 1

In the gram stain process, gram-positive cells primarily hold the dye crystal violet.

The answer is option ⇒a) Crystal violet

Step-by-step explanation:

The Gram stain procedure involves different steps. Firstly, crystal violet is applied to the bacterial cells, staining them purple. Then, a mordant called Gram's iodine is added, which sets the stain by forming a complex with crystal violet.

Next, decolorization with ethanol or acetone/ethanol solution is done, which removes the stain from gram-negative cells but not from gram-positive cells. Finally, a secondary stain called safranin is added, which counterstains the decolorized cells pink.

The answer is option ⇒a) Crystal violet