Final answer:
Key Characteristics of L. monocytogenes are b) Negative, Positive, Positive, Negative
Step-by-step explanation:
The correct combination of characteristics for *Listeria monocytogenes* is b) Negative, Positive, Positive, Negative.
- **Catalase:** L. monocytogenes is catalase-negative, meaning it does not produce catalase enzyme.
- **Esculin:** L. monocytogenes is esculin-positive. It hydrolyzes esculin in the presence of bile, leading to a characteristic blackening of the medium.
- **CAMP Test:** L. monocytogenes shows a positive reaction in the CAMP test. The CAMP factor produced by L. monocytogenes enhances the hemolytic activity of *Streptococcus agalactiae* (Group B Streptococcus) when the two are streaked perpendicularly on a blood agar plate.
- **Motility on Wet Mount:** L. monocytogenes is motile, exhibiting a characteristic tumbling or darting motility under wet mount examination.
Understanding these key characteristics is essential for the identification of *Listeria monocytogenes*, a bacterium known for causing the foodborne illness listeriosis. Differentiating bacterial species based on such traits is a fundamental aspect of clinical microbiology and plays a crucial role in diagnosing infections and implementing appropriate treatments.