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Bile solubility pos.

A) Sensitive to optochin
B) VP pos.
C) PYR neg.
D) Grows in 6.5% NaCl broth

User Mralexhay
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Final answer:

The question deals with the identification and characteristics of bacteria based on their growth in selective media with bile salts and high NaCl concentrations, relevant to the field of microbiology.

Step-by-step explanation:

The question pertains to Biology, specifically microbiology, as it involves the identification and characteristics of bacteria with regards to various conditions such as bile solubility, growth in the presence of NaCl, and the use of bile esculin agar as a selective medium. Bacteria that grow in the presence of bile are often those that can survive in the gastrointestinal tract, such as enterococci.

Bile salts and crystal violet in media inhibit the growth of most Gram-positive, non-enteric bacteria, making it a selective agent for isolating certain bacterial types. Moreover, bile esculin agar is used for the isolation and identification of enterococci because they are not only resistant to bile but can also hydrolyze esculin. Bacteria capable of growing in media with high concentrations of NaCl, such as 6.5% NaCl broth or mannitol salt agar, are known as halotolerant or halophilic, and this property is important for distinguishing Staphylococcus aureus from other species. The study of bacteria in varied environments provides insight into their adaptability and potential pathogenicity, important for understanding infections and foodborne illnesses.

User Dorin Botan
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