Final answer:
The fifth step of Clean Needle Technique required in acupuncture settings is to wear gloves during the procedure to ensure a sterile environment and safety.
Step-by-step explanation:
The fifth step of Clean Needle Technique that MUST be followed in all acupuncture settings is B) Wear gloves during the procedure. This is a critical component of maintaining a sterile environment and protecting both the patient and practitioner from possible infection. It is also aligned with general safety protocols in medical settings where handling of sharps and potential exposure to bloodborne pathogens are concerns, just as personnel who handle contaminated specimens or equipment must wear appropriate protective gear.