Final answer:
In spreadsheet applications, the range of cells from A1 through C5 is correctly represented by the notation 'A1:C5'.
Step-by-step explanation:
The correct answer to the question of what represents the range of cells from A1 through C5 in spreadsheet software like Microsoft Excel or Sheets is C. A1:C5. This notation is used to reference all cells in a block or array that starts at cell A1 and ends at cell C5, which includes all cells in the rows 1 through 5 and the columns A through C.