Question

The concentration of enzyme for each experiment was 5.0 μM. What is kcat for the reaction at pH 4.5 with NO chloride added when Compound 3 is the substrate?

A. 2.5 × 10-2 s-1
B. 1.3 × 102 s-1
C. 5.3 × 103 s-1
D. 7.0 × 105 s-1

Answer 1

To calculate the kcat value for the reaction at pH 4.5 with NO chloride added when Compound 3 is the substrate, we need specific information such as the initial rate of the reaction and the concentration of the product formed.

Step-by-step explanation:

The kcat value for the reaction at pH 4.5 with NO chloride added when Compound 3 is the substrate can be calculated using the Michaelis-Menten equation. The kcat value represents the turnover number, which is the number of substrate molecules converted to product per enzyme molecule per unit of time.

1. Given that the concentration of enzyme is 5.0 μM, we can use this information to calculate the initial rate of the reaction using the Michaelis-Menten equation.
2. Once we have the initial rate, we can divide it by the concentration of enzyme to calculate the kcat value.

Therefore, to calculate the kcat value for the given reaction, we need specific information such as the initial rate of the reaction and the concentration of the product formed.