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Outline the methods of direct and indirect selection of mutants

User RossBille
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Final answer:

Direct selection of mutants involves detecting the desired gene or its protein product. Indirect selection involves designing mutants to alter conserved sequences in a gene or protein and generating them using primers.

Step-by-step explanation:

Direct selection of mutants involves selecting mutants based on the detection of the desired gene or its protein product in the culture plate itself. This can be done using marker selection procedures, where a specific marker is used to identify the presence of the gene or protein.

Indirect selection of mutants involves designing mutants to alter the sequence or structural elements of a conserved sequence in a gene or protein. This can be done by designing primers to generate mutants with specific mutations.

For example, in the case of identifying conserved elements in a sensor, mutants can be designed to alter these conserved elements and then generated using primers. This allows for the study of the impact of these mutations on the function of the sensor.

User Danypata
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