Final answer:
Immersion oil is specifically designed for use with 100x objective lenses in microscopy to maximize resolution and magnification, and it should not be used with other lenses to prevent microscope damage. the correct option is D) 100x .
Step-by-step explanation:
Immersion oil is typically used with 100x objective lenses on a microscope:
Using immersion oil with an oil immersion objective is crucial for achieving maximum resolution and to effectively observe specimens at high magnifications. When using a microscope, light rays from the specimen enter the objective lens.
The use of an immersion medium such as oil, which has a refractive index (n = 1.51) closer to that of glass, minimizes light refraction and allows more light rays into the objective lens, thus increasing resolution. The oil immersion lens can achieve a total magnification of 1000x, with a resolution of 0.2 µm, making it an essential tool for studying small cells such as most prokaryotic cells. It's important to only use immersion oil with the designated 100x oil immersion lens to avoid damaging the microscope, and to clean the oil from the lenses after each use.