Final answer:
To handle HD APIs prior to sterilization, a sterile field should be used to prevent contamination. Holding bottles or tubes at an angle and passing the opening through a flame can minimize the risk of airborne contaminants. Proper pipette practice is also important for compounding sterile HDs.
Step-by-step explanation:
HD APIs, or High-Dose Active Pharmaceutical Ingredients, should be handled prior to sterilization in a controlled and sterile environment to minimize the risk of contamination. This is typically done in a designated area called a sterile field, which is maintained using proper aseptic techniques to prevent sepsis. Before compounding sterile HDs, tubes or bottles containing the APIs should be held at an angle during the transfer process to minimize the potential for airborne contaminants to enter the opening. Additionally, passing the opening of the tube or bottle briefly through a flame before and after the transfer process can discourage the entry of airborne contaminants into the sterile liquid.
Prior to compounding sterile HDs, it's important to have proper pipette practice. This involves understanding the divisions on the pipette and what volume each mark represents. Practice drawing up and releasing water using a sterile graduated pipette with a pipette aid (pipump). Once comfortable, practice using aseptic techniques to pipette a sterile liquid from a capped media bottle.