Final answer:
DNA sequencing cannot be used to determine the physical location of a gene on a chromosome. It sequences the order of nucleotides, but does not reveal the gene's chromosomal locale. Other methods like In Situ Hybridization or Deletion Mapping are appropriate for this purpose.
Step-by-step explanation:
The physical location of a gene on a chromosome cannot be determined using DNA sequencing from the options provided.
DNA sequencing is a powerful tool for determining the order of nucleotides within a DNA molecule, but it does not inherently provide information about the gene's specific physical location on a given chromosome.
The other methods listed - In Situ Hybridization, Somatic Cell Hybridization, Three-Point Genetic Cross, and Deletion Mapping - are all used to ascertain the physical positioning of genes on chromosomes.
To elaborate:
- In Situ Hybridization uses labeled DNA or RNA probes to locate specific DNA regions on chromosomes by hybridizing to complementary sequences.
- Somatic Cell Hybridization involves the fusion of cells from different species to determine gene location based on the presence or absence of species-specific traits.
- Three-Point Genetic Cross is used to determine the order and relative distances between linked genes on a chromosome through recombination frequencies.
- Deletion Mapping utilizes known deletions within a chromosome to pinpoint the location of a gene by observing phenotypic changes.
Among the methods for creating physical maps, which detail the physical distance between genetic markers, are:
- Cytogenetic mapping - Using a microscope to analyze stained sections of chromosomes to determine the approximate distance between genetic markers.
- Radiation hybrid mapping - Breaking DNA into fragments with radiation, a technique unaffected by recombination frequency.
- Sequence mapping - Employing DNA sequencing technology to create detailed physical maps, which is a separate process from using that technology to ascertain a gene's specific chromosome location.