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What are the two ways to count microbial growth?

A) Absorbance and fluorescence
B) Direct and indirect
C) Hemocytometer and Coulter counter
D) Spectrophotometry and turbidity

User RukTech
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Final answer:

Microbial growth can be counted by direct methods using a microscope and counting chambers, or indirectly through spectrophotometry by measuring turbidity that estimates cell density.

Step-by-step explanation:

The two ways to count microbial growth are direct and indirect methods. Direct methods involve counting individual cells or colonies, typically using a microscope and specialized counting chambers, such as a Petroff-Hausser chamber or a hemocytometer. Indirect methods do not count the cells directly but instead estimate cell density by measuring the impact of the cellular population on a physical property, such as turbidity or light absorbance.

Spectrophotometry is an indirect method where a spectrophotometer measures the turbidity of a liquid culture by determining how much light passes through the suspension. Greater bacterial cell density results in higher turbidity, reducing light transmission and increasing absorbance.

User Jody Bleyle
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