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A leucine auxotroph requires the addition of _________ to minimal medium for growth.

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Final answer:

A leucine auxotroph requires the addition of leucine to a minimal medium to grow, as it cannot synthesize this amino acid due to a genetic mutation.

Step-by-step explanation:

A leucine auxotroph is a nutritional mutant that requires leucine for growth because it has a loss-of-function mutation in a gene responsible for the biosynthesis of leucine.

In the context of the scenario provided, a leucine auxotroph would require the addition of leucine to a minimal medium to support its growth.

This is because the auxotroph is unable to synthesize leucine on its own due to a genetic mutation affecting the pathway that normally produces this amino acid.

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