Question

A restriction enzyme that makes single stranded ends that will hydrogen bond to other DNA fragments cut with the same restriction enzyme produces _____ ends?

1) Sticky
2) Blunt
3) Double-stranded
4) Circular

Answer 1

The correct option is 1. A restriction enzyme that makes single stranded ends that will hydrogen bond to other DNA fragments cut with the same restriction enzyme produces sticky ends.

Step-by-step explanation:

A restriction enzyme that makes single stranded ends that will hydrogen bond to other DNA fragments cut with the same restriction enzyme produces sticky ends. These sticky ends are capable of coming back together by hydrogen bonding with complementary overhangs on other DNA fragments, allowing the fragments to be joined together.

The process of forming hydrogen bonds between complementary sequences on single strands to form double-stranded DNA is called annealing, and DNA ligase is the enzyme that permanently joins the DNA fragments when the sticky ends come together.