Question

What are the steps involved in DNA replication?

1) Separation of DNA strands, addition of primers, use of DNA polymerase to produce the second strand of DNA
2) Addition of primers, separation of DNA strands, use of DNA polymerase to produce the second strand of DNA
3) Use of DNA polymerase to produce the second strand of DNA, separation of DNA strands, addition of primers
4) Use of DNA polymerase to produce the second strand of DNA, addition of primers, separation of DNA strands

Answer 1

The steps involved in DNA replication include separation of the dsDNA template or strand separation, annealing of the primers to the template DNA, and DNA synthesis catalyzed by a polymerase.

Step-by-step explanation:

1. Separation of the dsDNA template or strand separation
2. Annealing of the primers to the template DNA (they form Watson-Crick base pairs) to initiate DNA synthesis
3. DNA synthesis catalyzed by a polymerase

The general steps involved in DNA replication include unwinding of the DNA helix, opening up the replication forks, coating the DNA with single-strand binding proteins, preventing supercoiling with topoisomerase, synthesizing RNA primers with primase, and adding nucleotides to the primer using DNA polymerase.

Elongation of both the leading and lagging strands continues, and the RNA primers are removed by exonuclease activity.

The gaps are filled with DNA nucleotides, and the fragments are sealed by DNA ligase.