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DNA fingerprinting is a method of identification based on amino acid sequence analysis. True or False?

User Avakar
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Final answer:

DNA fingerprinting is a forensic technique involving the analysis of unique regions of DNA, not amino acid sequences, and uses methods like PCR, restriction enzymes, and gel electrophoresis to generate identifiable patterns. It is false that DNA fingerprinting is based on amino acid sequence analysis.

Step-by-step explanation:

The statement that DNA fingerprinting is based on amino acid sequence analysis is false. DNA fingerprinting, also known as DNA typing or genetic fingerprinting, is a forensic technique that focuses on analyzing the unique regions of DNA that vary between individuals.

The process involves extracting DNA from samples such as blood, skin, saliva, or semen, amplifying it using the Polymerase Chain Reaction (PCR), and then cutting the DNA into fragments with restriction enzymes.

These fragments are separated by gel electrophoresis, generating a pattern of bands that is compared between samples to identify individuals or determine relationships.

DNA fingerprinting leverages variations in the nucleotide sequences, known as polymorphisms, rather than amino acid sequences.

A reference to amino acids would be applicable to protein fingerprinting or profiling, which is a different technique concerned with identifying protein structures through methods like two-dimensional gel electrophoresis.

Restriction Fragment Length Polymorphism (RFLP) is one of the earliest techniques used in DNA fingerprinting. It utilizes the fact that individuals have DNA with different restriction enzyme cleavage sites, creating a unique banding pattern that can be visualized after gel electrophoresis.

These characteristics are inheritable, and forensic scientists can compare the banding patterns from different DNA samples to help solve crimes or establish paternity.

User Smaclell
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