Final answer:
The punctuation to begin a multi-line comment is option 2) /* used in languages like C, C++, and Java. // signifies single-line comments, and # is used in scripting languages like Python.
Step-by-step explanation:
The punctuation used to begin a multi-line comment in many programming languages is option 2) /*. When creating multi-line comments, you typically use /* to start the comment and */ to end the comment. Single-line comments are often denoted by // in languages like C, C++, or Java, while # is used for single-line comments in scripting languages like Python. The %% symbol is not commonly used to denote comments in programming.