Final answer:
The principle of lambda red recombination is a technique used in biology to introduce changes in an organism's DNA. It involves using a bacteriophage called lambda red to recombine DNA sequences and create targeted mutations or insert new genes. This technique has various applications in genetic research.
Step-by-step explanation:
The principle of lambda red recombination refers to a technique used in biological research to introduce changes in the DNA of an organism. It is based on the ability of a bacteriophage called lambda red to recombine DNA sequences. By using this technique, scientists can create targeted mutations or insert new genes into the DNA of an organism.
The process of lambda red recombination involves using DNA sequences called homology arms that are similar to the target sequence of the DNA. These homology arms guide the lambda red enzyme to the target sequence, where it can replace or modify the DNA. This technique has many applications in genetic research, such as creating genetically modified organisms or studying gene function.
For example, let's say scientists want to modify a specific gene in a mouse to study its function. They can design homology arms that are complementary to the target gene and introduce them along with the lambda red enzyme into the mouse's cells. The lambda red enzyme will then recombine the DNA, resulting in the desired modification of the gene.