Final answer:
Samples with signal readings higher than the highest standard will need to be diluted to ensure accurate and reliable readings within the linear range of an assay for quantitative analysis.
Step-by-step explanation:
When samples with signal readings are over the linear range, which is higher than the highest standard, they will need to be diluted to bring the concentration within the linear range of the assay being used. This is necessary to ensure that the readings are accurate and reliable. In analytical chemistry, maintaining samples within the linear range of an assay is crucial for quantitative analysis, as the detector's response is directly proportional to the analyte concentration only within this range. If a sample is too concentrated, the response may plateau, leading to inaccuracies. Therefore, dilution is a standard practice in labs to calibrate instrument readings for precise quantification.