Final answer:
The statement that an increased volume of cytoplasm is insufficient to cause mitotic spindle formation is not a legitimate conclusion from the control experiment.
Step-by-step explanation:
The statement that is not a legitimate conclusion from the control experiment is B: An increased volume of cytoplasm is insufficient to cause mitotic spindle formation.
In the control experiment, the researchers withdrew cytoplasm from a mid-meiotic oocyte and injected it into a pre-meiotic oocyte.
This induced premature meiosis in the immature oocyte.
Therefore, the experiment showed that the cytoplasm contains a chemical that causes the cell to lose its nuclear membrane, condense its chromatin into chromosomes, and enter meiosis.
Since the injection of cytoplasm was successful in inducing meiosis, the conclusion that an increased volume of cytoplasm is insufficient to cause mitotic spindle formation is not legitimate.