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What information regarding an enzyme-catalyzed reaction is obtained in a plot of the inverse of the initial velocities against the inverse of the corresponding substrate concentrations?

(A) 1/Vmax and 1/Km
(B) 1/V and 1/[S]
(C) Vmax and Km
(D) V and [S]

1 Answer

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Final answer:

A Lineweaver-Burk plot of 1/v against 1/[S] provides 1/Vmax and 1/Km in enzyme-catalyzed reactions.

Step-by-step explanation:

The information regarding an enzyme-catalyzed reaction obtained in a plot of the inverse of the initial velocities (1/v) against the inverse of the corresponding substrate concentrations (1/[S]) is (A) 1/Vmax and 1/Km. This type of plot is known as a Lineweaver-Burk plot, which is a double reciprocal plot. It linearizes the hyperbolic relationship between substrate concentration and reaction velocity described by the Michaelis-Menten equation, making it easier to determine Vmax and Km by extrapolating to the y-intercept and x-intercept, respectively.

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