Final answer:
FRAP cannot be used to isolate cells for cell culture; it's a technique for measuring molecular diffusion in cells, unlike FACS and centrifugation which are used for cell sorting and isolation.
Step-by-step explanation:
The technique that cannot be used to isolate cells for cell culture is FRAP (Fluorescence Recovery After Photobleaching). On the other hand, techniques such as FACS (Fluorescence-Activated Cell Sorting) and centrifugation are commonly utilized in cell isolation.
FACS is a highly sophisticated method that sorts cells based on their fluorescent characteristics after they have been labeled with specific antibodies or dyes. It is particularly useful for isolating live cells from dead ones or sorting cells into different subpopulations. Centrifugation is another method that uses centrifugal force to separate cells based on their density, which is a standard step in many cell fractionation procedures, where cells are broken up and their organelles are isolated for further analysis.
FRAP, however, does not serve the purpose of cell isolation for culture. It is a technique used to measure the rate of diffusion or transport of a fluorescent molecule within a region of the cell after a portion of the fluorescence has been bleached by intense light.