Final answer:
Subcellular organelles cannot be isolated using light microscopy, as it is a visualization technique, not a physical separation method. Instead, differential and density-gradient centrifugation or antibody-specific isolation are used for this purpose.
Step-by-step explanation:
The subcellular organelles cannot be isolated using light microscopy. Light microscopy can visualize cells and their structures but is not capable of physically separating organelles for isolation.Differential centrifugation, density-gradient centrifugation, and the use of an antibody against a specific type of organelle are all techniques that can be used to isolate subcellular organelles. Differential centrifugation separates cellular components by mass during the spin process. Density-gradient centrifugation separates organelles based on density in various media gradients.
Antibody-based methods utilize the specificity of antibodies to capture and isolate target organelles from mixed populations. All of these methods facilitate in-depth studies of cell structure and functions, particularly those related to a cell's stress responses, protein modifications such as phosphorylation, and can be used to prepare organelles for further microscopic examination or biochemical testing to confirm their identities.Although light microscopy is a powerful tool for visualizing cell structures, it cannot be used to physically isolate organelles from the cell.