Question

Normally, the resolution limit of human eyes, a conventional light microscope and TEM is __, __, and__.

A. 2 mm, 2 µm and 10 nm.
B. 0.2 mm , 0.2 µm and 0.1 nm.
C. 10 µm, 1 µm and 0.1 µm.
D. 100 µm, 10 µm and 0.1 µm.

Answer 1

The resolution limit of human eyes is approximately 0.2 mm, for a conventional light microscope it's 0.2 µm due to light diffraction limits, and for a Transmission Electron Microscope (TEM) it's much higher at 0.1 nm due to its use of electron beams.

Step-by-step explanation:

The resolution limit of human eyes, a conventional light microscope, and a Transmission Electron Microscope (TEM) are different due to their varying capabilities in distinguishing fine details. The human eye normally has a resolution limit of approximately 0.2 mm. A conventional light microscope, like those used in college biology labs, has a resolution limit of about 0.2 µm, as they are limited by diffraction of light waves and thus cannot observe objects smaller than this size with clarity. The oil immersion lens is particularly notable for achieving a high resolution limit of 0.2 µm with a magnification of 1000X. On the other hand, TEMs have an extremely high resolution of about 0.1 nm, and they use an electron beam instead of light, allowing for greater detail to be resolved.

Therefore, the correct answer to the question is: Normally, the resolution limit of human eyes, a conventional light microscope, and TEM is 0.2 mm, 0.2 µm, and 0.1 nm.