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The antigen-antibody interaction has found applications in all the following EXCEPT

A. Western blot
B. Antibody-affinity chromatography
C. Fluorescence-activated cell sorting (FACS)
D. Fluorescence recovery after photobleaching (FRAP)

1 Answer

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Final answer:

The correct option is C. The antigen-antibody interaction has applications in Western blot, Antibody-affinity chromatography, and Fluorescence-activated cell sorting (FACS), but not Fluorescence recovery after photobleaching (FRAP).

Step-by-step explanation:

The antigen-antibody interaction is utilized in all the options mentioned except for Fluorescence recovery after photobleaching (FRAP). Let's discuss the applications of antigen-antibody interaction in the other options:

  1. Western blot: In this technique, specific proteins are identified using antibodies after gel electrophoresis. It helps in detecting and analyzing proteins of interest.
  2. Antibody-affinity chromatography: This method uses antibodies coupled to a solid support to isolate and purify proteins based on their affinity for the antibody. It helps in separating and purifying specific proteins from a mixture.
  3. Fluorescence-activated cell sorting (FACS): FACS utilizes fluorescently labeled antibodies to sort and analyze cells based on their specific surface markers or antigens. It is commonly used in immunology and cell biology research.

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