Question

Which of the following information can be gained from determining the primary structure of an enzymatically active protein?

A. The amino acids in the active site can be positively identified
B. The molecular weight of the protein can be accurately established
C. The temperature at which thermal inactivation will occur can be accurately established
D. An estimate of Km can be calculated
E. An estimate of Vmax can be calculated.

Answer 1

Determining the primary structure of an enzymatically active protein can help identify amino acids in the active site, calculate E. estimates of Km and Vmax.

Step-by-step explanation:

The primary structure of an enzymatically active protein can provide the following information:

1. The amino acids in the active site can be positively identified. The active site is the region where the substrate binds for the reaction, and amino acid residues within the active site participate in the catalytic action of the enzyme.
2. An estimate of Km can be calculated. Km is a measure of the affinity between the enzyme and its substrate, and the primary structure can provide insights into the interactions between the enzyme and substrate.
3. An estimate of Vmax can be calculated. Vmax is the maximum rate of the enzyme-catalyzed reaction, and understanding the primary structure can help determine the efficiency of the enzyme's catalytic activity.