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What is abortive cycling?

A. When RNAP transcribes 2-9 nt, then restarts again and does not leave the promoter

B. When RNAP pools the template strand into its active site

C. When RNA polymerase hops on and off the DNA molecule searching for the promoter sequence

D. When free RNAP core loses the sigma subunit and clears the promoter

1 Answer

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Final answer:

Abortive cycling in transcription is the process by which RNA polymerase produces short RNA fragments without proceeding to full-length transcription, often occurring multiple times before successful RNA strand synthesis.

Step-by-step explanation:

Abortive cycling refers to an early phase of transcription where RNA polymerase (RNAP) synthesizes short RNA oligonucleotides of around 2-9 nucleotides and restarts transcription repeatedly without leaving the promoter. Essentially, RNA synthesis begins but stalls after a few nucleotides, leading to the dissociation of the short RNA segments and re-initiation by the RNA polymerase. This phenomenon can occur multiple times before a stable RNA-DNA hybrid is formed, and elongation of the RNA transcript proceeds successfully.

Detailed inspections of transcription mention that RNA polymerase also goes through a process known as termination. Termination can happen via a rho-independent mechanism, which is triggered by specific sequences in the DNA template that cause the formation of a hairpin structure in the mRNA, resulting in the dissociation of the RNA polymerase and release of the mRNA transcript. This termination is characterized by sequences rich in C-G nucleotides that form a stable hairpin, followed by a region rich in A-T nucleotides that forms a weak bond with the mRNA, leading to the release of the RNA transcript.

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