Question

What is the function of the RNAP Rudder/Lid domain?

A. Changes its conformation with each cycle of new nucleotide addition and keeps in contact with the growing RNA strand

B. Limits the length of RNA/DNA hybrid; separates nascent RNA chain from DNA template as it exits through the exit pore

C. Causes DNA bend; bending of DNA helps melt the strands and flip bases in the template strand to be accessible

D. Clamp is initially out of position; after DNA has melted, it gets repositioned to keep DNA in the active site tighter

Answer 1

The RNAP rudder/lid domain is responsible for limiting the RNA/DNA hybrid length and separating the nascent RNA from the DNA template during transcription.

Step-by-step explanation:

The function of the RNA polymerase (RNAP) rudder/lid domain is B. Limits the length of RNA/DNA hybrid; separates nascent RNA chain from DNA template as it exits through the exit pore. The rudder/lid domain plays a crucial role in the transcription process by maintaining the correct RNA and

DNA strand lengths within the RNA polymerase complex, and ensures the proper separation of the RNA transcript from the DNA template as the newly synthesized RNA exits the enzyme.