Question

Which of the following is NOT the activity of Mfd protein?

A. It recruits the uvrAB proteins and directs repair to the damaged template strand.

B. It binds to stalled RNA polymerase.

C. It degrades RNA polymerase

D. It displaces RNA polymerase.

Answer 1

The Mfd protein does not degrade RNA polymerase. Its role in the Transcription Coupled Repair pathway is to bind to stalled RNA polymerase, displace it, and recruit UvrAB proteins for DNA repair.

Step-by-step explanation:

The activity that is NOT associated with the Mfd protein is C. It degrades RNA polymerase.

The Mfd protein, also known as mutation frequency decline protein, is involved in the Transcription Coupled Repair pathway. It performs several functions:

• It binds to RNA polymerase that has stalled at a site of DNA damage,
• It displaces the stalled RNA polymerase from the DNA to allow access to the damaged site,
• It recruits the UvrAB proteins to the damaged site, which then initiates repair of the damaged template strand.

Therefore, the correct answer regarding the activity not associated with Mfd protein is that It degrades RNA polymerase, which Mfd does not do. Instead, it displaces it to allow the repair mechanisms to access the DNA lesion.