Question

In the context of replication in a medium containing phosphorus-32 (32P), what happens in the initial sample removed immediately after transfer, and what occurs after one and two rounds of replication in the 32P-containing medium regarding the incorporation of 32P into the DNA strands?

Answer 1

In the initial sample after transfer, parental DNA labeled with 32P is present. After one round of replication, new DNA strands incorporating 32P are synthesized. After two rounds of replication, three bands are observed in the DNA sample.

Step-by-step explanation:

In the context of replication in a medium containing phosphorus-32 (32P), the initial sample removed immediately after transfer would contain parental DNA labeled with 32P. After one round of replication, the newly synthesized DNA strands would also incorporate 32P, resulting in two bands in the DNA sample: one band at the position of the parental DNA and another at the position of the newly synthesized DNA. After two rounds of replication, the DNA sample would show three bands: one at the position of the parental DNA, one at the position of the newly synthesized DNA after one round of replication, and another at the position of the newly synthesized DNA after two rounds of replication.