Question

On the microscope, the _______________ objective is recommended for blood smear evaluation.

a. 40X high dry
b. 40X oil immersion
c. 10X
d. 100X oil immersion

Answer 1

The ideal objective for blood smear evaluation on a microscope is the 100X oil immersion objective, which provides a 1000X total magnification for observing the detailed structures of blood components.

Step-by-step explanation:

On the microscope, the best objective for blood smear evaluation is the 100X oil immersion objective. This lens achieves a total magnification of 1000X with a resolution of 0.2 µm, allowing for a detailed analysis of cellular structures such as red blood cells, white blood cells, and platelets.

Using oil immersion increases the resolution by allowing more light rays to enter the objective lens, as it matches the refractive index of glass, minimizing the loss of light due to refraction. The oil immersion objective is specifically designed for this kind of detailed observation, and it is critical to use a drop of oil on the immersion cover glass to achieve the highest resolution and clarity.

Key points to remember when using the oil immersion objective include cleaning the lens with appropriate lens paper, never using the coarse focusing knob with high-power objectives, and carefully applying and cleaning off the oil to avoid damaging the microscope.

Answer 2

For blood smear evaluation, the 100X oil immersion objective is recommended because it provides high magnification and resolution, essential for detailed analysis of cell structures. This objective achieves 1000X magnification with a resolution of 0.2 µm, and the use of immersion oil is necessary to enhance image clarity.

Step-by-step explanation:

When evaluating a blood smear under a microscope, the objective that is recommended is the 100X oil immersion objective. The oil immersion lens is specifically designed to achieve high magnification with superior resolution, essential for detailed analysis of cell structures, such as red and white blood cells in a blood smear. It increases the resolving power, which is the microscope's ability to distinguish two adjacent structures as separate entities, enhancing the clarity and detail of the image.

The 100X oil immersion lens reaches a magnification of 1000 times, providing a resolution of 0.2 micrometers. Staining the blood smear with selective dyes also helps in increasing the contrast, allowing for the clearer visualization of cellular components. The use of immersion oil is crucial with the 100X objective as it prevents refraction of light rays, which could otherwise reduce image quality. Always remember to clean the oil from the lens after use, as oil can damage the lenses over time if not removed.

Overall, the 100X oil immersion objective is the preferred lens for blood smear evaluation, as it provides the necessary magnification and resolution to conduct a detailed analysis of cells.