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What material(s) are used for site-directed mutagenesis?

User Alentejo
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Final answer:

Site-directed mutagenesis involves making specific changes to the DNA sequence. The QuickChange mutagenesis kit is commonly used for this technique.

Step-by-step explanation:

Site-directed mutagenesis is a technique used to make specific changes to the DNA sequence of a gene. In this process, researchers use primers and PCR to introduce mutations at desired locations in the DNA. The QuickChange mutagenesis kit is commonly used for site-directed mutagenesis, as it allows scientists to specify exactly where DNA base changes will occur.

The QuickChange process involves designing primers that are complementary to the target DNA sequence. These primers contain the desired mutations and are used in PCR to amplify the DNA. The amplified DNA can then be cloned and further analyzed.

User DudeOnRock
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