Question

You have a piece of circular DNA that can be cut by the restriction nucleases EcoRI, HindIII, and NotI, as indicated in Figure Q10-6.Which of the following statements is false?

(a) One piece of DNA will be obtained when this DNA is cut by NotI.
(b) A piece of DNA that cannot be cut by EcoRI will be obtained by cutting this DNA with both NotI and HindIII.
(c) Two DNA fragments that cannot be cut by HindIII will be obtained when this DNA is cut by EcoRI and NotI.
(d) Two DNA fragments of unequal size will be created when this DNA is cut by both HindIII and EcoRI.

Answer 1

The question revolves around the specific cuts made by restriction enzymes on circular DNA and which of the presented statements about the resulting fragments is false.

Step-by-step explanation:

The subject of this question involves circular DNA and the interaction with different restriction enzymes like EcoRI, HindIII, and NotI. These enzymes are used in recombinant DNA technology to cut DNA at specific sites, commonly resulting in DNA fragments with either sticky ends or blunt ends. EcoRI and HindIII, for instance, produce sticky ends, which can base pair with complementary DNA fragments to form recombinant DNA molecules.

Conversely, blunt end cutting enzymes, like NotI, cut DNA at specific sites without overhangs, thus creating blunt ends. The false statement among the options provided is likely to be related to the misunderstandings of how these enzymes cut and the fragments they produce.