Final answer:
The most suitable method to assess levels of expression of the target protein in different cell types is western blot analysis, which uses antibodies to specifically bind to the protein of interest and detect its presence and quantity.
Step-by-step explanation:
The most suitable method to assess levels of expression of your target protein in different cell types would be western blot analysis.
Western blot analysis is a technique that uses antibodies to specifically bind to the protein of interest. After performing protein gel electrophoresis, the protein antigens in the gel are transferred to a membrane and then exposed to antibodies. The presence and quantity of the target protein can be detected using secondary antibodies coupled to enzymes or fluorophores.
Gel filtration chromatography, gel electrophoresis, and ion-exchange chromatography are other methods commonly used in protein analysis.