Question

You are interested in a single-stranded DNA molecule that contains the following sequence:Which molecule can be used as a probe that will hybridize to your sequence of interest?

(a) 5′-GATTGCAT-3′
(b) 5′-TACGTTAG-3′
(c) 5′-CTAACGTA-3′
(d) 5′-ATGCAATC-3′

Answer 1

To identify the complementary DNA strand for the sequence 5'-ATTCCGGATCGA-3', we match each nucleotide with its complement based on the base pairing rules. The correct complementary sequence is 5'-TAAGGCCTAGCT-3', which corresponds to answer option A.

Step-by-step explanation:

If a DNA strand contains the sequence 5'-ATTCCGGATCGA-3', we are looking for the sequence of the complementary strand. To find this, we will apply the base pairing rules, which state that adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). Thus, for the given sequence, we would match each nucleotide with its complement: A to T, T to A, C to G, and G to C.

Given this rule, we can deduce the complementary strand for each nucleotide:

A becomes T

T becomes A

C becomes G

G becomes C

By following this pattern, the complementary strand for the given sequence would be 5'-TAAGGCCTAGCT-3'. Therefore, the correct answer is A. 5'-TAAGGCCTAGCT-3'.