Question

In which light microscope technique does a stain absorb energy from ultraviolet light and

glow with a bright color or colors against a dark background? The method is often used to localize
specific molecules within a cell.
A) dark field microscopy
B) bright field microscopy
C) phase contrast microscopy
D) fluorescence microscopy
E) polarization microscopy

Answer 1

D) Fluorescence microscopy is the technique that uses stains to absorb UV light and emit visible light, highlighting specific cell molecules.

Step-by-step explanation:

The light microscope technique where a stain absorbs energy from ultraviolet light and glows with a bright color or colors against a dark background is known as fluorescence microscopy.

This method is utilized to localize specific molecules within a cell. Fluorescence microscopy operates by using stains or dyes, which are chemical compounds that attach to specific cell components.

These dyes include fluorochromes like Texas red, FITC, DAPI, and acridine orange.

These fluorochromes absorb the high-energy ultraviolet light and emit it as visible light, marking the location of the target molecules. Thus, the correct answer to this question is D) fluorescence microscopy.