54.7k views
4 votes
Which light microscope technique below is ideally suited for high contrast specimens like

stained tissue sections?
A) dark field microscopy
B) bright field microscopy
C) phase contrast microscopy
D) fluorescence microscopy
E) polarization microscopy

1 Answer

4 votes

Final answer:

Bright field microscopy is the ideal technique for viewing high contrast specimens, such as stained tissue sections, due to the enhanced contrast provided by the staining. The correct option is B.

Step-by-step explanation:

The light microscope technique that is ideally suited for high contrast specimens like stained tissue sections is bright field microscopy. This microscopy method uses transmitted light to illuminate the specimen from below, allowing for the visualization of the specimen based on the differential absorption of light by various regions.

When tissue sections are stained, the dye provides distinct contrast, enabling detailed observation of cellular structures. Bright field microscopy is thus favored for these samples as the staining enhances contrast and allows for easier differentiation between different cellular components.

In contrast, techniques like dark field microscopy and phase-contrast microscopy are better suited for live, unfixed, and unstained specimens, as they increase contrast by other means.

Fluorescence microscopy is also a powerful technique; however, it is typically reserved for specimens that are labeled with fluorescent dyes. The correct option is B.

User Julien Simon
by
8.0k points