Question

Of the following steps associated with translation, which does not involve hydrolysis of GTP?

A) aminoacylation of tRNA
B) formation of the initiation complex
C) binding of the aminoacyl tRNA to the codon at the A site
D) translocation of the ribosome
E) release of polypeptide

Answer 1

Among the steps of translation, the release of the polypeptide is the one that does not involve hydrolysis of GTP, as it is triggered by stop codons and facilitated by release factors.

Step-by-step explanation:

The process of translation in cells involves multiple steps where the hydrolysis of GTP (guanosine triphosphate) provides the necessary energy for various activities. During aminoacylation of tRNA, ATP, not GTP, is hydrolyzed to charge the tRNA with an amino acid.

However, steps such as the formation of the initiation complex, binding of the aminoacyl tRNA to the codon at the A site, and translocation of the ribosome all involve hydrolysis of GTP. The release of the polypeptide does not involve the hydrolysis of GTP; this step is triggered by a stop codon and facilitated by release factors. Therefore, the step that does not involve hydrolysis of GTP is the release of the polypeptide.

The step in translation that does not involve hydrolysis of GTP is the release of the polypeptide, which is instead triggered by stop codons and facilitated by release factors without the need for GTP hydrolysis.