Question

Target sequence of DNA fragment:

- 3'-CTAGATCCTAGGAT-5'
What is the sequence and polarity of an equivalent-length molecular probe used to detect this target sequence?

Answer 1

To detect the target DNA sequence 3'-CTAGATCCTAGGAT-5', the equivalent-length molecular probe will have a complementary and antiparallel sequence, which is 5'-GATCTAGGATCCTA-3'.

Step-by-step explanation:

The sequence and polarity of an equivalent-length molecular probe used to detect a target sequence of DNA must be complementary and antiparallel to the target.

Given the target sequence 3'-CTAGATCCTAGGAT-5', we can deduce that the probe sequence will be 5'-GATCTAGGATCCTA-3' as the bases of the probe will form specific hydrogen bonds with the target sequence (adenine pairs with thymine, and cytosine pairs with guanine).

Answer 2

The equivalent-length molecular probe for the DNA target sequence 3'-CTAGATCCTAGGAT-5' is 5'-CTAGATCCTAGGAT-3', designed to be complementary and antiparallel to the target sequence.

Step-by-step explanation:

The sequence and polarity of an equivalent-length molecular probe used to detect the given target sequence 3'-CTAGATCCTAGGAT-5' would be 5'-CTAGATCCTAGGAT-3'.

To create a probe for a specific DNA sequence, one must generate a strand that is complementary and antiparallel to the target.

This means that the nucleotides of the probe must pair up according to the base pairing rules: cytosine with guanine, and adenine with thymine.