Final answer:
Gel filtration chromatography is the technique that separates proteins based on size and shape, independently of their charge.
Step-by-step explanation:
The technique that separates proteins independently of their charge is Gel filtration chromatography. It separates proteins based on their size and shape, not their charge. Other methods mentioned, such as Native PAGE, Ion exchange chromatography, and Isoelectric focusing, all involve separation based on charge or a combination of charge and size.
Gel filtration chromatography, however, allows proteins to pass through a column filled with porous beads, where smaller proteins enter the pores and take longer to elute from the column, while larger proteins bypass the pores and elute more quickly. This method is thus effective for separating proteins without regard to their charge.