Question

In pulse-chase experiment, radioactive chemicals are added into a growth medium during?

a) during the pulse period
b) during the chase period
c) during both pulse and chase period
d) at the end of the experiment
e) when samples are taken for analysis

Answer 1

Radioactive chemicals are added to the growth medium both during the pulse and chase periods in a pulse-chase experiment. During the pulse period, the cells are exposed to radioactive chemicals for a short period of time. During the chase period, the cells are incubated in fresh medium without radioactive chemicals to allow them to continue their normal cellular processes.

Step-by-step explanation:

In a pulse-chase experiment, radioactive chemicals are added to a growth medium during both the pulse and chase periods. During the pulse period, the cells are exposed to radioactive chemicals for a short period of time. This allows the cells to incorporate the radioactive chemicals into their molecules.

During the chase period, the cells are then incubated in fresh medium without radioactive chemicals to allow them to continue their normal cellular processes while the radioactive molecules are chased and diluted within the cell.