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On what surface is a specimen for an electron microscope supported when it is placed into

the electron beam?
A) a glass slide
B) a plastic slide
C) a metal specimen grid
D) a diamond grid
E) a very strong magnetic field

1 Answer

4 votes

Final answer:

A specimen for an electron microscope is supported on a metal specimen grid. This is particularly crucial for TEM, where the specimen must be thin enough for electrons to transmit through it, revealing detailed internal structures.

Step-by-step explanation:

When placing a specimen into the electron beam of an electron microscope, the specimen is supported on a metal specimen grid. This is essential because the specimen needs to be very thin, especially in the case of a Transmission Electron Microscope (TEM), to allow electrons to pass through it. Preparing a specimen for a TEM involves ensuring the specimen is thin (20-100 nm thick), dehydrated, and sometimes stained with electron-dense materials to enhance opacity and thus contrast.

In contrast to Scanning Electron Microscopes (SEM), which render surface characteristics by reflecting a beam of electrons across a specimen that has usually been coated with a metal like gold, TEMs transmit electrons through the specimen, allowing us to view internal cellular structures with magnifications up to 100 million times the size of the original object.

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