Final answer:
A replica is the metal-carbon construct viewed in an electron microscope after the original freeze-fracture methodology specimen has been removed.The correct option is B.
Step-by-step explanation:
In the freeze-fracture methodology, the metal-carbon construct that is viewed under the electron microscope after the tissue template has been thawed, removed, and discarded is known as a replica. The technique involves splitting a frozen sample to reveal structures such as cell membranes or proteins, and then coating the fracture surface with a thin layer of metal followed by carbon. This metal-carbon layer forms a replica of the surface which is then examined using electron microscopy, providing detailed images of the sample's ultrastructure without the presence of the original biological material.