Question

Which electron microscope technique involves striking a frozen specimen with a knife

edge creating cracks in the ice of the tissue block that spread from the point of contact and interact with
tissue?
A) positive staining
B) negative staining
C) freeze-fracture replication
D) shadow casting
E) freeze-etch

Answer 1

Freeze-fracture replication is the electron microscope technique that involves striking a frozen specimen with a knife edge and creating cracks in the ice of the tissue block that spread and interact with tissue. The correct option is c.

Step-by-step explanation:

The technique that involves striking a frozen specimen with a knife edge and creating cracks in the ice of the tissue block that spread and interact with tissue is called freeze-fracture replication.

During freeze-fracture replication, the frozen specimen is fractured, and the fractured surface is exposed to a vacuum.

This technique allows for the visualization of internal structures and the study of membrane organization.

Some examples of freeze-fracture replication images include the detection of cell membrane proteins and the examination of cellular junctions. The correct option is c.