Final answer:
A laser scanning confocal microscope uses a finely focused laser beam to illuminate and create high-resolution images of the specimen, which can then be constructed into a three-dimensional image. The correct option is D.
Step-by-step explanation:
Laser scanning confocal microscopy is an advanced imaging technique used to increase image clarity and resolution. In a confocal microscope, a finely focused laser beam is used to illuminate the specimen.
This laser scans multiple z-planes successively to produce numerous two-dimensional, high-resolution images at various depths, which can then be constructed into a three-dimensional image by a computer.
The use of fluorescent dyes and the narrow aperture in confocal microscopy eliminates light that is not from the focus plane, enhancing the contrast and clarity of the images.
High magnification illumination with reflected light is normally achieved by using a laser light. The carefully controlled laser light does not result in the degradation of the specimen, which is crucial for live cell imaging. The correct option is D.