Question

The limit of resolution of the light microscope is approximately 200 nm. However, over

the last few years, some complex optical techniques have been developed that allow investigators to
localize fluorescently labeled proteins at resolutions down to the _________.
A) hundreds on millimeters
B) tens of nanometers
C) hundreds of nanometers
D) tens of picometers
E) tens of micrometers

Answer 1

Advanced optical techniques allow investigators to localize fluorescently labeled proteins at resolutions down to the a. tens of nanometers, which exceeds the traditional 200 nm resolution limit of light microscopes.

Step-by-step explanation:

The student has asked about the enhanced resolving power of optical microscopes due to advanced imaging techniques. The limit of resolution of a traditional light microscope is approximately 200 nanometers (nm). Some recent complex optical techniques, such as super-resolved fluorescence microscopy, have enabled researchers to localize fluorescently labeled proteins at much higher resolutions. This advanced microscopic technology significantly surpasses the traditional diffractive limit and has enabled resolutions down to the tens of nanometers, marked improvement over conventional optical microscopy.

The complex optical techniques developed in recent years allow investigators to localize fluorescently labeled proteins at resolutions down to the tens of nanometers. These techniques, known as super-resolved fluorescence microscopy, go beyond the limit of resolution of traditional light microscopes (approximately 200 nm) by circumventing the diffraction limit. By controlling the molecules emitting light in a specimen, finer details can be observed, providing high-resolution images with greater clarity and accuracy.