27.9k views
2 votes
What type of apparatus does one need to quantify the DNA produced during real-time PCR?

a) Spectrophotometer
b) Fluorometer
c) Gel electrophoresis
d) Thermocycler

1 Answer

2 votes

Final answer:

A fluorometer is necessary to measure and quantify the DNA produced during real-time PCR by monitoring the fluorescence emitted by a dye that intercalates with the double-stranded DNA.

Step-by-step explanation:

To quantify the DNA produced during real-time PCR, also known as quantitative PCR or qPCR, the type of apparatus needed is a fluorometer. A fluorometer is equipped to monitor the fluorescence emitted by a fluorescent dye that gets incorporated into the DNA as it is being synthesized during the PCR reaction. This fluorescence is directly proportional to the amount of double-stranded DNA produced in the reaction. Therefore, by measuring this fluorescence in real-time, one can quantify the DNA produced.

User Fractor
by
8.1k points