Question

A typical PCR run consists of between ______ cycles.
a) 100
b) 150
c) 200
d) 250

Answer 1

A typical PCR run consists of approximately 25-40 cycles, each doubling the number of double-stranded target DNA copies. The cycle consists of three steps: denaturation, annealing, and extension. This results in an exponential amplification of the desired sequence.

Step-by-step explanation:

A typical PCR run consists of approximately 25-40 cycles. Each cycle consists of three steps: denaturation, annealing, and extension. The denaturation step separates the double-stranded template DNA strands, the annealing step allows the primers to attach to the template strands, and the extension step facilitates the Taq polymerase to extend the polynucleotides on the primers and complete the polymerization. Each cycle doubles the number of double-stranded target DNA copies, resulting in an exponential amplification of the desired sequence.