Question

How can we deal with cooperative/non-cooperative?

a) Increase temperature
b) Alter pH
c) Use inhibitors
d) All of the above

Answer 1

We can deal with cooperative or non-cooperative enzymes by altering factors such as temperature, pH, and adding inhibitors. Each of these factors affects enzymes differently, potentially either increasing or decreasing their activity. Chaperones and feedback mechanisms also play a role in regulating enzyme activity.

Step-by-step explanation:

Dealing with cooperative or non-cooperative enzymes involves understanding how various environmental factors affect their activity. Enzyme activity can be influenced by factors such as changes in pH, temperature, and the presence of inhibitors. To modify enzyme activity, one could:

• Increase temperature - Enzymes typically function within a specific temperature range. High temperatures may lead to the denaturation of the protein.
• Alter pH - Each enzyme has an optimal pH at which it functions most efficiently. Altering the pH can disrupt enzyme activity.
• Use inhibitors - Inhibitors can bind to the active or allosteric site of an enzyme, reducing its activity.

These methods can be used to alter how enzymes interact with substrates and regulate metabolic pathways. A key component in cellular function, proteins called chaperones, can assist in the proper folding and functioning of other proteins, protecting them from stress, such as high temperatures or altered pH levels. Feedback mechanisms, including end product inhibition, are another way to control enzyme-mediated pathways.