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When using an Abbe Retometer to determine the retive index of a liquid, how much sample should be used:

a) Small quantity
b) Large quantity
c) No specific quantity
d) Varies by liquid viscosity

User Hobenkr
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1 Answer

5 votes

Final answer:

A small quantity of a sample is typically sufficient for using an Abbe Refractometer to find the refractive index of a liquid. The amount needed is just enough to cover the measurement surface, similar to the principle of using a burette for precise volume measurements in titrations.

Step-by-step explanation:

When using an Abbe Refractometer to determine the refractive index of a liquid, the amount of sample that should be used can vary. However, a common practice would be to use just enough sample to cover the refractometer's glass surface where the measurement is taken. This typically represents a small quantity of the sample.

For precise measurements with devices like micropipettes or a volumetric glass pipette, users may contribute amounts ranging from 2-20 μl, 20-200 μl, up to 3.00 ml, depending on the sample and the measurement requirements. When comparing to other lab equipment, such as a UV/vis spectrophotometer that might use a standard quartz cuvette with about 3 ml of liquid, the key is to use the minimum amount needed to obtain an accurate reading without wasting the sample.

Volume measurements are critical in many scientific procedures like titrations, where a burette is used, allowing for very precise fluid dispense down to 0.01 mL. Similarly, for an Abbe Refractometer, you'd only need enough liquid to form a good contact with the glass, ensuring the accuracy of the refractive index measurement.

User NikoNyrh
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